7eyk

X-ray diffraction
1.38Å resolution

Crystal structure of Escherichia coli ppnP-Selenomethionine derived

Released:
Source organism: Escherichia coli K-12
Entry authors: Wen Y, Wu BX

Function and Biology Details

Reactions catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
2'-deoxyuridine + phosphate = uracil + 2-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrimidine/purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.47 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C037 (Residues: 1-94; Coverage: 100%)
Gene names: JW0382, b0391, ppnP, yaiE
Sequence domains: Pyrimidine/purine nucleoside phosphorylase

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P62
Unit cell:
a: 59.658Å b: 59.658Å c: 52.652Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.151 0.169
Expression system: Escherichia coli