7ewm

X-ray diffraction
2.9Å resolution

Native crystal structure of S. cerevisiae Csn12 in complex with Thp3 and Sem1

Released:
Entry authors: Kuang Z, Niu L

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein THP3 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 33.96 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12049 (Residues: 186-470; Coverage: 61%)
Gene names: THP3, YP9499.03c, YPR045C
Sequence domains: SAC3/GANP family
Cop9 signalosome complex subunit 12 Chain: B
Molecule details ›
Chain: B
Length: 423 amino acids
Theoretical weight: 49.56 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47130 (Residues: 1-423; Coverage: 100%)
Gene names: CSN12, J1860, YJR084W
Sequence domains: PCI domain
26S proteasome complex subunit SEM1 Chain: C
Molecule details ›
Chain: C
Length: 89 amino acids
Theoretical weight: 10.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94742 (Residues: 1-89; Coverage: 100%)
Gene names: DSH1, SEM1, YDR363W-A
Sequence domains: DSS1/SEM1 family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P3221
Unit cell:
a: 115.575Å b: 115.575Å c: 126.825Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.222 0.251
Expression system: Escherichia coli BL21(DE3)