› 7euo

Electron Microscopy
2.9Å resolution

The structure of formyl peptide receptor 1 in complex with Gi and peptide agonist fMLF

Released:
DOI: 10.2210/pdb7euo/pdb
PDB entry 7euo coloured by chain, front view.
PDB entry 7euo coloured by chain, side view.
PDB entry 7euo coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for recognition of N-formyl peptides as pathogen-associated molecular patterns.
Chen G, Wang X, Liao Q, Ge Y, Jiao H, Chen Q, Liu Y, Lyu W, Zhu L, van Zundert GCP, Robertson MJ, Skiniotis G, Du Y, Hu H, Ye RD
Nat Commun 13 5232 (2022)
PMID: 36064945
Related structures: EMD-31323

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210810 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
fMet-Leu-Phe receptor Chain: R
Molecule details ›
Chain: R
Length: 378 amino acids
Theoretical weight: 41.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21462 (Residues: 1-350; Coverage: 100%)
Gene name: FPR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 357 amino acids
Theoretical weight: 39.29 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 269 amino acids
Theoretical weight: 28.81 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Peptide Agonist fMLF Chain: C
Molecule details ›
Chain: C
Length: 3 amino acids
Theoretical weight: 438 Da
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CLR 2 x CLR
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-31323
Expression systems:
  • Spodoptera frugiperda
  • Trichoplusia ni
  • Not provided

Quick links

Citations

4 review citations

Developmental and homeostatic signaling transmitted by the G-protein coupled receptor FPR2.
Chen et al. (2023)
3 more

1 mention without citation

Cryo-EM structure of the human chemerin receptor 1-Gi protein complex bound to the C-terminal nonapeptide of chemerin.
Wang et al. (2023)