› 7enc

Electron Microscopy
4.13Å resolution

TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)

Released:
DOI: 10.2210/pdb7enc/pdb
PDB entry 7enc coloured by chain, front view.
PDB entry 7enc coloured by chain, side view.
PDB entry 7enc coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the human Mediator and Mediator-bound preinitiation complex.
Chen X, Yin X, Li J, Wu Z, Qi Y, Wang X, Liu W, Xu Y
Science 372 (2021)
PMID: 33958484
Related structures: EMD-31207

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + H(2)O = ADP + phosphate
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 76-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236786 (preferred)
Entry contents:
68 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (70 distinct):
Mediator of RNA polymerase II transcription subunit 1 Chain: a
Molecule details ›
Chain: a
Length: 1581 amino acids
Theoretical weight: 168.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15648 (Residues: 1-1581; Coverage: 100%)
Gene names: ARC205, CRSP1, CRSP200, DRIP205, DRIP230, MED1, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2
Sequence domains: Mediator of RNA polymerase II transcription subunit 1
Mediator of RNA polymerase II transcription subunit 31 Chain: m
Molecule details ›
Chain: m
Length: 131 amino acids
Theoretical weight: 15.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y3C7 (Residues: 1-131; Coverage: 100%)
Gene names: CGI-125, MED31, SOH1
Sequence domains: SOH1
Mediator of RNA polymerase II transcription subunit 4 Chain: d
Molecule details ›
Chain: d
Length: 270 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NPJ6 (Residues: 1-270; Coverage: 100%)
Gene names: ARC36, DRIP36, HSPC126, MED4, VDRIP
Sequence domains: Vitamin-D-receptor interacting Mediator subunit 4
Mediator of RNA polymerase II transcription subunit 6 Chain: f
Molecule details ›
Chain: f
Length: 246 amino acids
Theoretical weight: 28.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75586 (Residues: 1-246; Coverage: 100%)
Gene names: ARC33, MED6
Sequence domains: MED6 mediator sub complex component
Mediator of RNA polymerase II transcription subunit 7 Chain: g
Molecule details ›
Chain: g
Length: 233 amino acids
Theoretical weight: 27.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O43513 (Residues: 1-233; Coverage: 100%)
Gene names: ARC34, CRSP9, MED7
Sequence domains: MED7 protein
Mediator of RNA polymerase II transcription subunit 8 Chain: h
Molecule details ›
Chain: h
Length: 268 amino acids
Theoretical weight: 29.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96G25 (Residues: 1-268; Coverage: 100%)
Gene name: MED8
Sequence domains: Mediator of RNA polymerase II transcription complex subunit 8
Mediator of RNA polymerase II transcription subunit 18 Chain: r
Molecule details ›
Chain: r
Length: 208 amino acids
Theoretical weight: 23.69 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BUE0 (Residues: 1-208; Coverage: 100%)
Gene name: MED18
Sequence domains: Med18 protein
Mediator of RNA polymerase II transcription subunit 20 Chain: t
Molecule details ›
Chain: t
Length: 212 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H944 (Residues: 1-212; Coverage: 100%)
Gene names: MED20, TRFP
Sequence domains: TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Mediator of RNA polymerase II transcription subunit 10 Chain: j
Molecule details ›
Chain: j
Length: 135 amino acids
Theoretical weight: 15.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BTT4 (Residues: 1-135; Coverage: 100%)
Gene names: L6, MED10, TRG17, TRG20
Sequence domains: Transcription factor subunit Med10 of Mediator complex
Mediator of RNA polymerase II transcription subunit 11 Chain: k
Molecule details ›
Chain: k
Length: 117 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9P086 (Residues: 1-117; Coverage: 100%)
Gene names: HSPC296, MED11
Sequence domains: Mediator complex protein
Mediator of RNA polymerase II transcription subunit 14 Chain: n
Molecule details ›
Chain: n
Length: 1454 amino acids
Theoretical weight: 160.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O60244 (Residues: 1-1454; Coverage: 100%)
Gene names: ARC150, CRSP2, CXorf4, DRIP150, EXLM1, MED14, RGR1, TRAP170
Sequence domains: Mediator complex subunit MED14
Mediator of RNA polymerase II transcription subunit 17 Chain: q
Molecule details ›
Chain: q
Length: 651 amino acids
Theoretical weight: 72.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NVC6 (Residues: 1-651; Coverage: 100%)
Gene names: ARC77, CRSP6, DRIP77, DRIP80, MED17, TRAP80
Sequence domains: Subunit 17 of Mediator complex
Mediator of RNA polymerase II transcription subunit 19 Chain: s
Molecule details ›
Chain: s
Length: 244 amino acids
Theoretical weight: 26.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: A0JLT2 (Residues: 1-244; Coverage: 100%)
Gene names: LCMR1, MED19
Sequence domains: Mediator of RNA pol II transcription subunit 19
Mediator of RNA polymerase II transcription subunit 21 Chain: u
Molecule details ›
Chain: u
Length: 144 amino acids
Theoretical weight: 15.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13503 (Residues: 1-144; Coverage: 100%)
Gene names: MED21, SRB7, SURB7
Sequence domains: Subunit 21 of Mediator complex
Mediator of RNA polymerase II transcription subunit 22 Chain: v
Molecule details ›
Chain: v
Length: 200 amino acids
Theoretical weight: 22.24 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15528 (Residues: 1-200; Coverage: 100%)
Gene names: MED22, SURF5
Sequence domains: Surfeit locus protein 5 subunit 22 of Mediator complex
Mediator of RNA polymerase II transcription subunit 26 Chain: z
Molecule details ›
Chain: z
Length: 600 amino acids
Theoretical weight: 65.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O95402 (Residues: 1-600; Coverage: 100%)
Gene names: ARC70, CRSP7, MED26
Sequence domains:
Mediator of RNA polymerase II transcription subunit 27 Chain: c
Molecule details ›
Chain: c
Length: 311 amino acids
Theoretical weight: 35.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P2C8 (Residues: 1-311; Coverage: 100%)
Gene names: CRSP34, CRSP8, MED27
Sequence domains: Mediator complex subunit 27
Mediator of RNA polymerase II transcription subunit 28 Chain: e
Molecule details ›
Chain: e
Length: 178 amino acids
Theoretical weight: 19.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H204 (Residues: 1-178; Coverage: 100%)
Gene names: EG1, FKSG20, MED28
Sequence domains: Mediator complex subunit 28
Mediator of RNA polymerase II transcription subunit 29 Chain: b
Molecule details ›
Chain: b
Length: 200 amino acids
Theoretical weight: 21.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NX70 (Residues: 1-200; Coverage: 100%)
Gene names: IXL, MED29
Sequence domains: Mediator complex subunit 29
Mediator of RNA polymerase II transcription subunit 30 Chain: l
Molecule details ›
Chain: l
Length: 178 amino acids
Theoretical weight: 20.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96HR3 (Residues: 1-178; Coverage: 100%)
Gene names: MED30, THRAP6, TRAP25
Sequence domains: Mediator complex subunit 30
Mediator of RNA polymerase II transcription subunit 15 Chain: o
Molecule details ›
Chain: o
Length: 788 amino acids
Theoretical weight: 86.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96RN5 (Residues: 1-788; Coverage: 100%)
Gene names: ARC105, CTG7A, MED15, PCQAP, TIG1, TNRC7
Sequence domains:
Mediator of RNA polymerase II transcription subunit 9 Chain: i
Molecule details ›
Chain: i
Length: 146 amino acids
Theoretical weight: 16.42 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NWA0 (Residues: 1-146; Coverage: 100%)
Gene names: MED25, MED9
Sequence domains: RNA polymerase II transcription mediator complex subunit 9
CDK-activating kinase assembly factor MAT1 Chain: 0
Molecule details ›
Chain: 0
Length: 309 amino acids
Theoretical weight: 35.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51948 (Residues: 1-309; Coverage: 100%)
Gene names: CAP35, MAT1, MNAT1, RNF66
Sequence domains:
Cyclin-dependent kinase 7 Chain: 8
Molecule details ›
Chain: 8
Length: 346 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P50613 (Residues: 1-346; Coverage: 100%)
Gene names: CAK, CAK1, CDK7, CDKN7, MO15, STK1
Sequence domains: Protein kinase domain
Cyclin-H Chain: 9
Molecule details ›
Chain: 9
Length: 323 amino acids
Theoretical weight: 37.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51946 (Residues: 1-323; Coverage: 100%)
Gene name: CCNH
Sequence domains:
General transcription factor IIH subunit 1 Chain: 1
Molecule details ›
Chain: 1
Length: 548 amino acids
Theoretical weight: 62.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P32780 (Residues: 1-548; Coverage: 100%)
Gene names: BTF2, GTF2H1
Sequence domains:
General transcription factor IIH subunit 2 Chain: 2
Molecule details ›
Chain: 2
Length: 395 amino acids
Theoretical weight: 44.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13888 (Residues: 1-395; Coverage: 100%)
Gene names: BTF2P44, GTF2H2
Sequence domains:
General transcription factor IIH subunit 3 Chain: 3
Molecule details ›
Chain: 3
Length: 308 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13889 (Residues: 1-308; Coverage: 100%)
Gene name: GTF2H3
Sequence domains: Transcription factor Tfb4
General transcription factor IIH subunit 4 Chain: 4
Molecule details ›
Chain: 4
Length: 462 amino acids
Theoretical weight: 52.25 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92759 (Residues: 1-462; Coverage: 100%)
Gene name: GTF2H4
Sequence domains:
General transcription factor IIH subunit 5 Chain: 5
Molecule details ›
Chain: 5
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6ZYL4 (Residues: 1-71; Coverage: 100%)
Gene names: C6orf175, GTF2H5, TTDA
Sequence domains: Transcription factor TFIIH complex subunit Tfb5
General transcription and DNA repair factor IIH helicase/translocase subunit XPB Chain: 6
Molecule details ›
Chain: 6
Length: 782 amino acids
Theoretical weight: 89.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P19447 (Residues: 1-782; Coverage: 100%)
Gene names: ERCC3, XPB, XPBC
Sequence domains:
General transcription and DNA repair factor IIH helicase subunit XPD Chain: 7
Molecule details ›
Chain: 7
Length: 760 amino acids
Theoretical weight: 87.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P18074 (Residues: 1-760; Coverage: 100%)
Gene names: ERCC2, XPD, XPDC
Sequence domains:
General transcription factor IIE subunit 1 Chain: EA
Molecule details ›
Chain: EA
Length: 439 amino acids
Theoretical weight: 49.52 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P29083 (Residues: 1-439; Coverage: 100%)
Gene names: GTF2E1, TF2E1
Sequence domains:
Transcription initiation factor IIE subunit beta Chain: EB
Molecule details ›
Chain: EB
Length: 291 amino acids
Theoretical weight: 33.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P29084 (Residues: 1-291; Coverage: 100%)
Gene names: GTF2E2, TF2E2
Sequence domains:
Transcription initiation factor TFIID subunit 1 Chain: DA
Molecule details ›
Chain: DA
Length: 1872 amino acids
Theoretical weight: 212.96 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P21675 (Residues: 1-1893; Coverage: 99%)
Gene names: BA2R, CCG1, CCGS, TAF1, TAF2A
Sequence domains:
Transcription initiation factor TFIID subunit 2 Chain: DB
Molecule details ›
Chain: DB
Length: 1199 amino acids
Theoretical weight: 137.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P1X5 (Residues: 1-1199; Coverage: 100%)
Gene names: CIF150, TAF2, TAF2B
Transcription initiation factor TFIID subunit 4 Chains: DD, Dd
Molecule details ›
Chains: DD, Dd
Length: 1085 amino acids
Theoretical weight: 110.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O00268 (Residues: 1-1085; Coverage: 100%)
Gene names: TAF2C, TAF2C1, TAF4, TAF4A, TAFII130, TAFII135
Sequence domains:
Transcription initiation factor TFIID subunit 5 Chains: DE, De
Molecule details ›
Chains: DE, De
Length: 800 amino acids
Theoretical weight: 86.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15542 (Residues: 1-800; Coverage: 100%)
Gene names: TAF2D, TAF5
Sequence domains:
Transcription initiation factor TFIID subunit 6 Chains: DF, Df
Molecule details ›
Chains: DF, Df
Length: 677 amino acids
Theoretical weight: 72.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49848 (Residues: 1-677; Coverage: 100%)
Gene names: TAF2E, TAF6, TAFII70
Sequence domains:
Transcription initiation factor TFIID subunit 7 Chain: DG
Molecule details ›
Chain: DG
Length: 349 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15545 (Residues: 1-349; Coverage: 100%)
Gene names: TAF2F, TAF7, TAFII55
Sequence domains: TAFII55 protein conserved region
Transcription initiation factor TFIID subunit 8 Chain: DH
Molecule details ›
Chain: DH
Length: 310 amino acids
Theoretical weight: 34.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7Z7C8 (Residues: 1-310; Coverage: 100%)
Gene names: TAF8, TAFII43, TBN
Sequence domains:
Transcription initiation factor TFIID subunit 9 Chains: DI, Di
Molecule details ›
Chains: DI, Di
Length: 264 amino acids
Theoretical weight: 29.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q16594 (Residues: 1-264; Coverage: 100%)
Gene names: TAF2G, TAF9, TAFII31
Sequence domains: Transcription initiation factor IID, 31kD subunit
Transcription initiation factor TFIID subunit 10 Chains: DJ, Dj
Molecule details ›
Chains: DJ, Dj
Length: 218 amino acids
Theoretical weight: 21.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q12962 (Residues: 1-218; Coverage: 100%)
Gene names: TAF10, TAF2A, TAF2H, TAFII30
Sequence domains: Transcription initiation factor TFIID 23-30kDa subunit
Transcription initiation factor TFIID subunit 12 Chains: DL, Dl
Molecule details ›
Chains: DL, Dl
Length: 161 amino acids
Theoretical weight: 17.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q16514 (Residues: 1-161; Coverage: 100%)
Gene names: TAF12, TAF15, TAF2J, TAFII20
Sequence domains: Transcription initiation factor TFIID subunit A
Transcription initiation factor TFIID subunit 3 Chain: Dc
Molecule details ›
Chain: Dc
Length: 929 amino acids
Theoretical weight: 103.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5VWG9 (Residues: 1-929; Coverage: 100%)
Gene name: TAF3
Sequence domains:
Transcription initiation factor TFIID subunit 11 Chain: Dk
Molecule details ›
Chain: Dk
Length: 211 amino acids
Theoretical weight: 23.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15544 (Residues: 1-211; Coverage: 100%)
Gene names: PRO2134, TAF11, TAF2I
Sequence domains: hTAFII28-like protein conserved region
Transcription initiation factor TFIID subunit 13 Chain: Dm
Molecule details ›
Chain: Dm
Length: 124 amino acids
Theoretical weight: 14.31 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15543 (Residues: 1-124; Coverage: 100%)
Gene names: TAF13, TAF2K, TAFII18
Sequence domains: Transcription initiation factor IID, 18kD subunit
Transcription initiation factor IIA subunit 2 Chain: DO
Molecule details ›
Chain: DO
Length: 109 amino acids
Theoretical weight: 12.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P52657 (Residues: 1-109; Coverage: 100%)
Gene names: GTF2A2, TF2A2
Sequence domains:
TATA-box-binding protein Chain: DP
Molecule details ›
Chain: DP
Length: 339 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P20226 (Residues: 1-339; Coverage: 100%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
TFIIA-a Chain: DQ
Molecule details ›
Chain: DQ
Length: 307 amino acids
Theoretical weight: 34.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Transcription initiation factor IIB Chain: BA
Molecule details ›
Chain: BA
Length: 316 amino acids
Theoretical weight: 34.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q00403 (Residues: 1-316; Coverage: 100%)
Gene names: GTF2B, TF2B, TFIIB
Sequence domains:
General transcription factor IIF subunit 2 Chain: FB
Molecule details ›
Chain: FB
Length: 249 amino acids
Theoretical weight: 28.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P13984 (Residues: 1-249; Coverage: 100%)
Gene names: GTF2F2, RAP30
Sequence domains:
RPB1 Chain: PA
Molecule details ›
Chain: PA
Length: 1970 amino acids
Theoretical weight: 217.39 KDa
Source organism: Sus scrofa
DNA-directed RNA polymerase subunit beta Chain: PB
Molecule details ›
Chain: PB
Length: 1174 amino acids
Theoretical weight: 134.04 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1TVZ5 (Residues: 78-1251; Coverage: 94%)
Sequence domains:
DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein Chain: PC
Molecule details ›
Chain: PC
Length: 275 amino acids
Theoretical weight: 31.44 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LCH3 (Residues: 1-275; Coverage: 100%)
Gene name: POLR2C
Sequence domains:
RNA polymerase Rpb4/RPC9 core domain-containing protein Chain: PD
Molecule details ›
Chain: PD
Length: 142 amino acids
Theoretical weight: 16.33 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A481BYI6 (Residues: 1-142; Coverage: 100%)
Sequence domains: RNA polymerase Rpb4
DNA-directed RNA polymerase II subunit E Chain: PE
Molecule details ›
Chain: PE
Length: 210 amino acids
Theoretical weight: 24.64 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VTX4 (Residues: 1-210; Coverage: 100%)
Gene name: POLR2E
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: PF
Molecule details ›
Chain: PF
Length: 127 amino acids
Theoretical weight: 14.48 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VEK9 (Residues: 1-127; Coverage: 100%)
Gene name: POLR2F
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase subunit Chain: PG
Molecule details ›
Chain: PG
Length: 172 amino acids
Theoretical weight: 19.31 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VKG7 (Residues: 1-172; Coverage: 100%)
Gene name: POLR2G
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: PH
Molecule details ›
Chain: PH
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LCB2 (Residues: 1-150; Coverage: 100%)
Gene name: POLR2H
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerase II subunit RPB9 Chain: PI
Molecule details ›
Chain: PI
Length: 125 amino acids
Theoretical weight: 14.54 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P60899 (Residues: 1-125; Coverage: 100%)
Gene name: POLR2I
Sequence domains:
RPB10 Chain: PJ
Molecule details ›
Chain: PJ
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Sus scrofa
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein Chain: PK
Molecule details ›
Chain: PK
Length: 117 amino acids
Theoretical weight: 13.31 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1RKE4 (Residues: 1-117; Coverage: 100%)
Gene name: POLR2J
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: PL
Molecule details ›
Chain: PL
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1TRS6 (Residues: 1-58; Coverage: 100%)
Gene name: POLR2K
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
General transcription factor IIF subunit 1 Chain: FA
Molecule details ›
Chain: FA
Length: 517 amino acids
Theoretical weight: 58.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P35269 (Residues: 1-517; Coverage: 100%)
Gene names: GTF2F1, RAP74
Sequence domains: Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Mediator of RNA polymerase II transcription subunit 23 Chain: w
Molecule details ›
Chain: w
Length: 1368 amino acids
Theoretical weight: 156.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9ULK4 (Residues: 1-1368; Coverage: 100%)
Gene names: ARC130, CRSP3, DRIP130, KIAA1216, MED23, SUR2
Sequence domains: Mediator complex subunit 23
Mediator of RNA polymerase II transcription subunit 24 Chain: x
Molecule details ›
Chain: x
Length: 989 amino acids
Theoretical weight: 110.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75448 (Residues: 1-989; Coverage: 100%)
Gene names: ARC100, CRSP4, DRIP100, KIAA0130, MED24, THRAP4, TRAP100
Sequence domains: Mediator complex subunit 24 N-terminal
Mediator of RNA polymerase II transcription subunit 16 Chain: p
Molecule details ›
Chain: p
Length: 841 amino acids
Theoretical weight: 93.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y2X0 (Residues: 1-828; Coverage: 94%)
Gene names: DRIP92, MED16, THRAP5
Sequence domains:
DNA (69-MER) Chain: X
Molecule details ›
Chain: X
Length: 69 nucleotides
Theoretical weight: 21.46 KDa
DNA (69-MER) Chain: Y
Molecule details ›
Chain: Y
Length: 69 nucleotides
Theoretical weight: 21.09 KDa

Ligands and Environments

3 bound ligands:
Ligand ZN 16 x ZN
Ligand SF4 1 x SF4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.13Å
Relevant EMDB volumes: EMD-31207
Expression system: Homo sapiens

Quick links

Citations

9 review citations

The Mediator complex as a master regulator of transcription by RNA polymerase II.
Richter et al. (2022)
8 more

7 mentions without citation

Assembly of RNA polymerase II transcription initiation complexes.
Farnung et al. (2022)
6 more