7ekb

X-ray diffraction
1.45Å resolution

Crystal structure of 4E10 modified with pyrene acetamide

Released:
Entry authors: Caaveiro JMM, Rujas E, Nieva JL

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab region of the heavy chain of broadly neutralizing antibody anti-HIV-1 4E10 Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 23.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Fab region of the light chain of the broadly neutralizing anti-HIV-1 antibody 4E10 Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
MPER region of the HIV-1 envelope glycoprotein protein gp41 Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 2.19 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2
Unit cell:
a: 158.109Å b: 44.89Å c: 86.041Å
α: 90° β: 113.68° γ: 90°
R-values:
R R work R free
0.121 0.12 0.15
Expression systems:
  • Escherichia coli BL21
  • Not provided