› 7ekb

X-ray diffraction
1.45Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209089 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab region of the heavy chain of broadly neutralizing antibody anti-HIV-1 4E10 Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 23.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Fab region of the light chain of the broadly neutralizing anti-HIV-1 antibody 4E10 Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Transmembrane protein gp41 Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 2.19 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: P04578 (Residues: 671-683; Coverage: 2%)
Gene name: env

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2
Unit cell:
a: 158.109Å b: 44.89Å c: 86.041Å
α: 90° β: 113.68° γ: 90°
R-values:
R R work R free
0.121 0.12 0.15
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

1 mention without citation

Focal accumulation of aromaticity at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV neutralization profile.
Rujas et al. (2021)