7ehn

X-ray diffraction
2.25Å resolution

Human MTHFD2 in complex with compound 21 and 9

Released:

Function and Biology Details

Reactions catalysed:
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
5,10-methylenetetrahydrofolate + NAD(+) = 5,10-methenyltetrahydrofolate + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13995 (Residues: 36-350; Coverage: 90%)
Gene names: MTHFD2, NMDMC
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P65
Unit cell:
a: 113.169Å b: 113.169Å c: 113.67Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.24
Expression system: Escherichia coli BL21(DE3)