› 7ehi

X-ray diffraction
1.69Å resolution

Crystal structure of covalent maltosyl-alpha-glucosidase intermediate

Released:
DOI: 10.2210/pdb7ehi/pdb
PDB 7ehi coloured by chain and viewed from the front.
PDB 7ehi coloured by chain and viewed from the side.
PDB 7ehi coloured by chain and viewed from the top.
Source organism: Weissella cibaria
Primary publication:
A GH13 α-glucosidase from Weissella cibaria uncommonly acts on short-chain maltooligosaccharides.
Wangpaiboon K, Laohawuttichai P, Kim SY, Mori T, Nakapong S, Pichyangkura R, Pongsawasdi P, Hakoshima T, Krusong K
Acta Crystallogr D Struct Biol 77 1064-1076 (2021)
PMID: 34342279

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
alpha glucosidase Chain: A
Molecule details ›
Chain: A
Length: 589 amino acids
Theoretical weight: 68.4 KDa
Source organism: Weissella cibaria
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand MES 2 x MES
Ligand GOL 2 x GOL
Ligand CA 1 x CA
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P4122
Unit cell:
a: 116.566Å b: 116.566Å c: 107.181Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links