› 7eeb

Electron Microscopy
2.9Å resolution

Structure of the CatSpermasome

Released:
DOI: 10.2210/pdb7eeb/pdb
PDB entry 7eeb coloured by chain, front view.
PDB entry 7eeb coloured by chain, side view.
PDB entry 7eeb coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a mammalian sperm cation channel complex.
Lin S, Ke M, Zhang Y, Yan Z, Wu J
Nature 595 746-750 (2021)
PMID: 34225353
Related structures: EMD-31076

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-236768 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (19 distinct):
Cation channel sperm-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 955 amino acids
Theoretical weight: 109.44 KDa
Source organism: Human betaherpesvirus 5
Expression system: Mus musculus
UniProt:
  • Canonical: Q91ZR5 (Residues: 1-686; Coverage: 100%)
Gene name: Catsper1
Sequence domains: Ion transport protein
Cation channel sperm-associated protein 2 Chain: B
Molecule details ›
Chain: B
Length: 588 amino acids
Theoretical weight: 68.65 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: A2ARP9 (Residues: 1-588; Coverage: 100%)
Gene name: Catsper2
Sequence domains: Ion transport protein
Cation channel sperm-associated protein 3 Chain: C
Molecule details ›
Chain: C
Length: 395 amino acids
Theoretical weight: 45.53 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q80W99 (Residues: 1-395; Coverage: 100%)
Gene name: Catsper3
Sequence domains: Ion transport protein
Cation channel sperm-associated protein 4 Chain: D
Molecule details ›
Chain: D
Length: 442 amino acids
Theoretical weight: 51.18 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q8BVN3 (Residues: 1-442; Coverage: 100%)
Gene name: Catsper4
Sequence domains: Ion transport protein
Cation channel sperm-associated auxiliary subunit beta Chain: E
Molecule details ›
Chain: E
Length: 1109 amino acids
Theoretical weight: 126.25 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: A2RTF1 (Residues: 1-1109; Coverage: 100%)
Gene name: Catsperb
Sequence domains:
Cation channel sperm-associated auxiliary subunit gamma 2 Chain: F
Molecule details ›
Chain: F
Length: 1145 amino acids
Theoretical weight: 131.55 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: C6KI89 (Residues: 1-1145; Coverage: 100%)
Gene names: Catsperg, Catsperg2
Sequence domains: Cation channel sperm-associated protein subunit gamma
Cation channel sperm-associated auxiliary subunit delta Chain: G
Molecule details ›
Chain: G
Length: 805 amino acids
Theoretical weight: 91.19 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: E9Q9F6 (Residues: 1-805; Coverage: 100%)
Gene names: Catsperd, Tmem146
Sequence domains: Cation channel sperm-associated protein subunit delta
Cation channel sperm-associated auxiliary subunit epsilon Chain: H
Molecule details ›
Chain: H
Length: 985 amino acids
Theoretical weight: 113.91 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P0DP43 (Residues: 1-985; Coverage: 100%)
Gene names: Catspere, Catspere1, Gm7068
Sequence domains: Cation channel sperm-associated protein subunit delta
Solute carrier organic anion transporter family member 6C1 Chain: L
Molecule details ›
Chain: L
Length: 706 amino acids
Theoretical weight: 79.06 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q8C0X7 (Residues: 1-706; Coverage: 100%)
Gene name: Slco6c1
Sequence domains:
Cation channel sperm-associated auxiliary subunit TMEM249 Chain: J
Molecule details ›
Chain: J
Length: 171 amino acids
Theoretical weight: 19.92 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: A0A2R8VHF7 (Residues: 1-171; Coverage: 100%)
Gene name: Tmem249
Sequence domains: Cation channel sperm-associated auxiliary subunit TMEM249
Cation channel sperm-associated auxiliary subunit TMEM262 Chain: M
Molecule details ›
Chain: M
Length: 116 amino acids
Theoretical weight: 13.49 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: D3Z338 (Residues: 1-116; Coverage: 100%)
Gene names: Catsperh, Tmem262
Unknown Chain: N
Molecule details ›
Chain: N
Length: 28 amino acids
Theoretical weight: 2.4 KDa
Source organism: Mus musculus
EF-hand calcium-binding domain-containing protein 9 Chain: I
Molecule details ›
Chain: I
Length: 216 amino acids
Theoretical weight: 26.17 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9DAM2 (Residues: 1-216; Coverage: 100%)
Gene name: Efcab9
Cation channel sperm-associated auxiliary subunit zeta Chain: K
Molecule details ›
Chain: K
Length: 194 amino acids
Theoretical weight: 22.78 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CQP8 (Residues: 1-194; Coverage: 100%)
Gene names: Catsperz, Tex40

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand NA 2 x NA
Ligand 9Z9 3 x 9Z9
Ligand NAG 9 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-31076
Expression system: Mus musculus

Quick links

Citations

10 review citations

Testicular aging, male fertility and beyond.
Dong et al. (2022)
9 more

4 mentions without citation

Kv Channel Ancillary Subunits: Where Do We Go from Here?
Abbott GW. (2022)
3 more