› 7edo

X-ray diffraction
2.7Å resolution

First insight into marsupial MHC I peptide presentation: immune features of lower mammals paralleled with bats

Released:
DOI: 10.2210/pdb7edo/pdb
PDB entry 7edo coloured by chain, front view.
PDB entry 7edo coloured by chain, side view.
PDB entry 7edo coloured by chain, top view.
Source organisms:
Primary publication:
Peptide Presentations of Marsupial MHC Class I Visualize Immune Features of Lower Mammals Paralleled with Bats.
Wang P, Yue C, Liu K, Lu D, Liu S, Yao S, Li X, Su X, Ren K, Chai Y, Qi J, Zhao Y, Lou Y, Sun Z, Gao GF, Liu WJ
J Immunol 207 2167-2178 (2021)
PMID: 34535575

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158316 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 362 amino acids
Theoretical weight: 40.83 KDa
Source organism: Trichosurus vulpecula
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95IT0 (Residues: 1-362; Coverage: 100%)
Gene name: Truv-UB
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
CYS-ASN-VAL-THR-LEU-ASN-TYR-PRO Chains: C, F
Molecule details ›
Chains: C, F
Length: 8 amino acids
Theoretical weight: 923 Da
Source organism: uncultured virus
Expression system: Not provided
Beta-2-microglobulin Chain: E
Molecule details ›
Chain: E
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: I23
Unit cell:
a: 193.633Å b: 193.633Å c: 193.633Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

New vistas unfold: Chicken MHC molecules reveal unexpected ways to present peptides to the immune system.
Halabi et al. (2022)
1 more