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Structural basis of assembly and torque transmission of the bacterial flagellar motor.

Tan J, Zhang X, Wang X, Xu C, Chang S, Wu H, Wang T, Liang H, Gao H, Zhou Y, Zhu Y
Cell 184 2665-2679.e19 (2021)
Related entries: 7cbl, 7cbm, 7cg0, 7cg4, 7cg7, 7cgb, 7cgo, 7e80, 7e81

Cited: 34 times
EuropePMC logo PMID: 33882274

Abstract

The bacterial flagellar motor is a supramolecular protein machine that drives rotation of the flagellum for motility, which is essential for bacterial survival in different environments and a key determinant of pathogenicity. The detailed structure of the flagellar motor remains unknown. Here we present an atomic-resolution cryoelectron microscopy (cryo-EM) structure of the bacterial flagellar motor complexed with the hook, consisting of 175 subunits with a molecular mass of approximately 6.3 MDa. The structure reveals that 10 peptides protruding from the MS ring with the FlgB and FliE subunits mediate torque transmission from the MS ring to the rod and overcome the symmetry mismatch between the rotational and helical structures in the motor. The LP ring contacts the distal rod and applies electrostatic forces to support its rotation and torque transmission to the hook. This work provides detailed molecular insights into the structure, assembly, and torque transmission mechanisms of the flagellar motor.

Reviews citing this publication (8)

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Articles citing this publication (26)

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  3. A multi-state dynamic process confers mechano-adaptation to a biological nanomachine. Wadhwa N, Sassi A, Berg HC, Tu Y. Nat Commun 13 5327 (2022)
  4. Genetic Analysis of the Salmonella FliE Protein That Forms the Base of the Flagellar Axial Structure. Hendriksen JJ, Lee HJ, Bradshaw AJ, Namba K, Chevance FFV, Minamino T, Hughes KT. mBio 12 e0239221 (2021)
  5. Structural and Functional Analysis of SsaV Cytoplasmic Domain and Variable Linker States in the Context of the InvA-SsaV Chimeric Protein. Xu J, Wang J, Liu A, Zhang Y, Gao X. Microbiol Spectr 9 e0125121 (2021)
  6. Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit. Hu H, Popp PF, Santiveri M, Roa-Eguiara A, Yan Y, Martin FJO, Liu Z, Wadhwa N, Wang Y, Erhardt M, Taylor NMI. Nat Commun 14 4411 (2023)
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  11. Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection. Sun M, Liu M, Shan H, Li K, Wang P, Guo H, Zhao Y, Wang R, Tao Y, Yang L, Zhang Y, Su X, Liu Y, Li C, Lin J, Chen XL, Zhang YZ, Shen QT. Sci Adv 9 eadd2796 (2023)
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  14. FlgV forms a flagellar motor ring that is required for optimal motility of Helicobacter pylori. Botting JM, Tachiyama S, Gibson KH, Liu J, Starai VJ, Hoover TR. PLoS One 18 e0287514 (2023)
  15. Loss of Flagella-Related Genes Enables a Nonflagellated, Fungal-Predating Bacterium To Strengthen the Synthesis of an Antifungal Weapon. Xiong D, Yang Z, He X, He W, Shen D, Wang L, Lin L, Murero A, Minamino T, Shao X, Qian G. Microbiol Spectr 11 e0414922 (2023)
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  18. Design and Motion Analysis of a Soft-Limb Robot Inspired by Bacterial Flagella. Ye C, Liu Z, Yu S, Fan Z, Wang Y. Biomimetics (Basel) 8 271 (2023)
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  20. In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography. Lai L, Cheung YW, Martinez M, Kixmoeller K, Palao L, Steimle S, Ho MC, Black BE, Lai EM, Chang YW. Methods Mol Biol 2646 211-248 (2023)
  21. Long-Time Dynamics of Selected Molecular-Motor Components Using a Physics-Based Coarse-Grained Approach. Liwo A, Pyrka M, Czaplewski C, Peng X, Niemi AJ. Biomolecules 13 941 (2023)
  22. Recent Advances in Modeling Membrane β-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies. Duncan AL, Gao Y, Haanappel E, Im W, Chavent M. Methods Mol Biol 2778 311-330 (2024)
  23. Response mechanisms to acid stress promote LF82 replication in macrophages. Yao T, Huang Y, Huai Z, Liu X, Liu X, Liu Y, Sun H, Pang Y. Front Cell Infect Microbiol 13 1255083 (2023)
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  25. The common origin and degenerative evolution of flagella in Actinobacteria. Zhu S, Sun X, Li Y, Feng X, Gao B. mBio e0252623 (2023)
  26. The protein interactome of the citrus Huanglongbing pathogen Candidatus Liberibacter asiaticus. Carter EW, Peraza OG, Wang N. Nat Commun 14 7838 (2023)


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