› 7e82

Electron Microscopy
3.3Å resolution

Cryo-EM structure of the flagellar rod with partial hook from Salmonella

Released:
DOI: 10.2210/pdb7e82/pdb
PDB 7e82 coloured by chain and viewed from the front.
PDB 7e82 coloured by chain and viewed from the side.
PDB 7e82 coloured by chain and viewed from the top.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Structural basis of assembly and torque transmission of the bacterial flagellar motor.
Tan J, Zhang X, Wang X, Xu C, Chang S, Wu H, Wang T, Liang H, Gao H, Zhou Y, Zhu Y
Cell 184 2665-2679.e19 (2021)
PMID: 33882274
Related structures: EMD-31008

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 67-mer (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Flagellar basal-body rod protein FlgG Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 260 amino acids
Theoretical weight: 27.78 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1J3 (Residues: 1-260; Coverage: 100%)
Gene names: STM1179, fla FVII, flaL, flgG
Sequence domains:
Flagellar basal-body rod protein FlgF Chains: a, b, c, d, e
Molecule details ›
Chains: a, b, c, d, e
Length: 251 amino acids
Theoretical weight: 26.12 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16323 (Residues: 1-251; Coverage: 100%)
Gene names: STM1178, fla FVI, flaX, flgF
Sequence domains:
Flagellar MS ring L2 Chains: 0, 1, 2, 3, 4
Molecule details ›
Chains: 0, 1, 2, 3, 4
Length: 15 amino acids
Theoretical weight: 1.29 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Flagellar M-ring protein Chains: 5, 6, 7, 8, 9
Molecule details ›
Chains: 5, 6, 7, 8, 9
Length: 21 amino acids
Theoretical weight: 2 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15928 (Residues: 311-331; Coverage: 4%)
Gene names: STM1969, fla AII.1, fla BI, fliF
Flagellar basal-body rod protein FlgC Chains: f, g, h, i, j, p
Molecule details ›
Chains: f, g, h, i, j, p
Length: 134 amino acids
Theoretical weight: 13.99 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1I7 (Residues: 1-134; Coverage: 100%)
Gene names: STM1175, fla FIII, flaW, flgC
Sequence domains:
Flagellar basal body rod protein FlgB Chains: k, l, m, n, o
Molecule details ›
Chains: k, l, m, n, o
Length: 138 amino acids
Theoretical weight: 15.15 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16437 (Residues: 1-138; Coverage: 100%)
Gene names: STM1174, fla FII, flbA, flgB
Sequence domains: Flagella basal body rod protein
Flagellar hook-basal body complex protein FliE Chains: q, r, s, t, u, v
Molecule details ›
Chains: q, r, s, t, u, v
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P26462 (Residues: 1-104; Coverage: 100%)
Gene names: STM1968, fla AI, flaN, fliE
Sequence domains: Flagellar hook-basal body complex protein FliE
Flagellar hook protein FlgE Chains: DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK
Molecule details ›
Chains: DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK
Length: 403 amino acids
Theoretical weight: 42.23 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1J1 (Residues: 1-403; Coverage: 100%)
Gene names: STM1177, fla FV, flaK, flgE
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-31008

Quick links