7e82

Electron Microscopy
3.3Å resolution

Cryo-EM structure of the flagellar rod with partial hook from Salmonella

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 67-mer (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Flagellar basal-body rod protein FlgG Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 260 amino acids
Theoretical weight: 27.78 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1J3 (Residues: 1-260; Coverage: 100%)
Gene names: STM1179, fla FVII, flaL, flgG
Sequence domains:
Flagellar basal-body rod protein FlgF Chains: a, b, c, d, e
Molecule details ›
Chains: a, b, c, d, e
Length: 251 amino acids
Theoretical weight: 26.12 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16323 (Residues: 1-251; Coverage: 100%)
Gene names: STM1178, fla FVI, flaX, flgF
Sequence domains:
Flagellar MS ring L2 Chains: 0, 1, 2, 3, 4
Molecule details ›
Chains: 0, 1, 2, 3, 4
Length: 15 amino acids
Theoretical weight: 1.29 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Flagellar M-ring protein Chains: 5, 6, 7, 8, 9
Molecule details ›
Chains: 5, 6, 7, 8, 9
Length: 21 amino acids
Theoretical weight: 2 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15928 (Residues: 311-331; Coverage: 4%)
Gene names: STM1969, fla AII.1, fla BI, fliF
Flagellar basal-body rod protein FlgC Chains: f, g, h, i, j, p
Molecule details ›
Chains: f, g, h, i, j, p
Length: 134 amino acids
Theoretical weight: 13.99 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1I7 (Residues: 1-134; Coverage: 100%)
Gene names: STM1175, fla FIII, flaW, flgC
Sequence domains:
Flagellar basal body rod protein FlgB Chains: k, l, m, n, o
Molecule details ›
Chains: k, l, m, n, o
Length: 138 amino acids
Theoretical weight: 15.15 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16437 (Residues: 1-138; Coverage: 100%)
Gene names: STM1174, fla FII, flbA, flgB
Sequence domains: Flagella basal body rod protein
Flagellar hook-basal body complex protein FliE Chains: q, r, s, t, u, v
Molecule details ›
Chains: q, r, s, t, u, v
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P26462 (Residues: 1-104; Coverage: 100%)
Gene names: STM1968, fla AI, flaN, fliE
Sequence domains: Flagellar hook-basal body complex protein FliE
Flagellar hook protein FlgE Chains: DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK
Molecule details ›
Chains: DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK
Length: 403 amino acids
Theoretical weight: 42.23 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1J1 (Residues: 1-403; Coverage: 100%)
Gene names: STM1177, fla FV, flaK, flgE
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-31008