› 7e4i

Electron Microscopy
3.05Å resolution

Cryo-EM structure of the yeast mitochondrial SAM-Tom40/Tom5/Tom6 complex at 3.0 angstrom

Released:
DOI: 10.2210/pdb7e4i/pdb
PDB entry 7e4i coloured by chain, front view.
PDB entry 7e4i coloured by chain, side view.
PDB entry 7e4i coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.
Wang Q, Guan Z, Qi L, Zhuang J, Wang C, Hong S, Yan L, Wu Y, Cao X, Cao J, Yan J, Zou T, Liu Z, Zhang D, Yan C, Yin P
Science 373 1377-1381 (2021)
PMID: 34446444
Related structures: EMD-30986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-147029 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Sorting assembly machinery 50 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 54.54 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Homo sapiens
UniProt:
  • Canonical: P53969 (Residues: 2-484; Coverage: 100%)
Gene names: N2802, SAM50, TOB55, YNL026W
Sequence domains: Omp85 superfamily domain
Sorting assembly machinery 35 kDa subunit Chain: B
Molecule details ›
Chain: B
Length: 329 amino acids
Theoretical weight: 37.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Homo sapiens
UniProt:
  • Canonical: P14693 (Residues: 1-329; Coverage: 100%)
Gene names: OMP85, SAM35, TOB38, TOM38, YHR083W
Sequence domains: SAM35, subunit of SAM coomplex
Sorting assembly machinery 37 kDa subunit Chain: C
Molecule details ›
Chain: C
Length: 351 amino acids
Theoretical weight: 40.5 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Homo sapiens
UniProt:
  • Canonical: P50110 (Residues: 1-327; Coverage: 100%)
Gene names: MAS37, PET3027, SAM37, TOM37, YM9796.13C, YMR060C
Sequence domains:
Mitochondrial import receptor subunit TOM40 Chain: D
Molecule details ›
Chain: D
Length: 406 amino acids
Theoretical weight: 43.95 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Homo sapiens
UniProt:
  • Canonical: P23644 (Residues: 2-387; Coverage: 100%)
Gene names: ISP42, MOM38, TOM40, YM8325.04, YMR203W
Sequence domains: Eukaryotic porin
Mitochondrial import receptor subunit TOM5 Chain: E
Molecule details ›
Chain: E
Length: 52 amino acids
Theoretical weight: 6.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Homo sapiens
UniProt:
  • Canonical: P80967 (Residues: 1-50; Coverage: 100%)
Gene names: MOM8A, TOM5, YPR133W-A
Sequence domains: Mitochondrial import receptor subunit or translocase
Mitochondrial import receptor subunit TOM6 Chain: F
Molecule details ›
Chain: F
Length: 62 amino acids
Theoretical weight: 6.48 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Homo sapiens
UniProt:
  • Canonical: P33448 (Residues: 2-61; Coverage: 98%)
Gene names: ISP6, TOM6, YOR045W
Sequence domains: Mitochondrial import receptor subunit Tom6, fungal

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.05Å
Relevant EMDB volumes: EMD-30986
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Crosstalk between Mitochondrial Protein Import and Lipids.
Hoffmann et al. (2022)
2 more

2 mentions without citation

Mechanistic insights into fungal mitochondrial outer membrane protein biogenesis.
Diederichs et al. (2022)
1 more