› 7e2s

X-ray diffraction
1.05Å resolution

Synechocystis GUN4 in complex with biliverdin IXa

Released:
DOI: 10.2210/pdb7e2s/pdb
PDB entry 7e2s coloured by chain, front view.
PDB entry 7e2s coloured by chain, side view.
PDB entry 7e2s coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4.
Hu JH, Chang JW, Xu T, Wang J, Wang X, Lin R, Duanmu D, Liu L
Protein Sci 30 2083-2091 (2021)
PMID: 34382282

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ycf53-like protein Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.49 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P72583 (Residues: 1-233; Coverage: 100%)
Gene name: sll0558
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand BLA 1 x BLA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 32.497Å b: 64.449Å c: 106.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.118 0.118 0.134
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Elucidating the origins of phycocyanobilin biosynthesis and phycobiliproteins.
Rockwell et al. (2023)
3 more

1 mention without citation

Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4.
Hu et al. (2021)