7dty

Electron Microscopy
2.98Å resolution

Structural basis of ligand selectivity conferred by the human glucose-dependent insulinotropic polypeptide receptor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209134 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Gastric inhibitory polypeptide receptor Chain: R
Molecule details ›
Chain: R
Length: 573 amino acids
Theoretical weight: 64.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P48546 (Residues: 22-421; Coverage: 90%)
Gene name: GIPR
Sequence domains:
Gastric inhibitory polypeptide Chain: P
Molecule details ›
Chain: P
Length: 42 amino acids
Theoretical weight: 4.99 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P09681 (Residues: 52-93; Coverage: 32%)
Gene name: GIP
Sequence domains: Peptide hormone
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.73 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04896 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 40.23 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 140 amino acids
Theoretical weight: 15.34 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.98Å
Relevant EMDB volumes: EMD-30860
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Escherichia coli