› 7dfl

Electron Microscopy
3.3Å resolution

Cryo-EM structure of histamine H1 receptor Gq complex

Released:
DOI: 10.2210/pdb7dfl/pdb
PDB entry 7dfl coloured by chain, front view.
PDB entry 7dfl coloured by chain, side view.
PDB entry 7dfl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the human histamine H1 receptor/Gq complex.
Xia R, Wang N, Xu Z, Lu Y, Song J, Zhang A, Guo C, He Y
Nat Commun 12 2086 (2021)
PMID: 33828102
Related structures: EMD-30665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211095 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Histamine H1 receptor Chain: R
Molecule details ›
Chain: R
Length: 487 amino acids
Theoretical weight: 55.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35367 (Residues: 1-487; Coverage: 100%)
Gene name: HRH1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(q) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 41.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 3-30, 162-163; Coverage: 9%)
  • Canonical: P50148 (Residues: 37-166, 169-359; Coverage: 89%)
Gene names: GAQ, GNAI1, GNAQ
Sequence domains: G-protein alpha subunit
scFv16 Chain: C
Molecule details ›
Chain: C
Length: 251 amino acids
Theoretical weight: 26.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand HSM 1 x HSM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-30665
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

Structural basis of GPCR coupling to distinct signal transducers: implications for biased signaling.
Seyedabadi et al. (2022)
8 more

17 mentions without citation

Histamine: A Bacterial Signal Molecule.
Krell et al. (2021)
16 more