X-ray diffraction
2.4Å resolution

Crystal structure of FtmOx1 Y224F mutation

Source organism: Aspergillus fumigatus AF210
Entry authors: Wang F, Gao YQ, Gan JH, Cao CY

Function and Biology Details

Reaction catalysed:
Fumitremorgin B + 2-oxoglutarate + 2 O(2) + AH(2) = verruculogen + succinate + CO(2) + H(2)O + A
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Verruculogen synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 299 amino acids
Theoretical weight: 33.76 KDa
Source organism: Aspergillus fumigatus AF210
Expression system: Escherichia coli
  • Canonical: Q4WAW9 (Residues: 1-291; Coverage: 100%)
Gene names: AFUA_8G00230, ftmF, ftmOx1
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 45.436Å b: 136.74Å c: 180.628Å
α: 90° β: 90° γ: 90°
R R work R free
0.218 0.216 0.263
Expression system: Escherichia coli