› 7dcu

X-ray diffraction
1.75Å resolution

Crystal structure of HSF2 DNA-binding domain in complex with 3-site HSE DNA (21 bp)

Released:
DOI: 10.2210/pdb7dcu/pdb
PDB entry 7dcu coloured by chain, front view.
PDB entry 7dcu coloured by chain, side view.
PDB entry 7dcu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of heat shock factor trimers bound to DNA.
Feng N, Feng H, Wang S, Punekar AS, Ladenstein R, Wang DC, Zhang Q, Ding J, Liu W
iScience 24 102951 (2021)
PMID: 34458700

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121210 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Heat shock factor protein 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 113 amino acids
Theoretical weight: 13.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q03933 (Residues: 7-112; Coverage: 20%)
Gene names: HSF2, HSTF2
Sequence domains: HSF-type DNA-binding
DNA (5'-D(*TP*GP*CP*GP*TP*TP*CP*TP*AP*GP*AP*AP*TP*AP*TP*TP*CP*GP*CP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.47 KDa
Source organism: Homo sapiens
Expression system: DNA satellites
DNA (5'-D(*AP*CP*CP*GP*CP*GP*AP*AP*TP*AP*TP*TP*CP*TP*AP*GP*AP*AP*CP*GP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Homo sapiens
Expression system: DNA satellites

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Unit cell:
a: 46.64Å b: 67.06Å c: 139.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.205
Expression systems:
  • Escherichia coli K-12
  • DNA satellites

Quick links

Citations

1 review citation

HIF1, HSF1, and NRF2: Oxidant-Responsive Trio Raising Cellular Defenses and Engaging Immune System.
Cyran et al. (2022)
4 other articles

2 mentions without citation

Structures of heat shock factor trimers bound to DNA.
Feng et al. (2021)
1 more