› 7d9t

Electron Microscopy
4.1Å resolution

Structure of human soluble guanylate cyclase in the cinciguat-bound inactive state

Released:
DOI: 10.2210/pdb7d9t/pdb
PDB entry 7d9t coloured by chain, front view.
PDB entry 7d9t coloured by chain, side view.
PDB entry 7d9t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation mechanism of human soluble guanylate cyclase by stimulators and activators.
Liu R, Kang Y, Chen L
Nat Commun 12 5492 (2021)
PMID: 34535643
Related structures: EMD-30620

Function and Biology Details

Reaction catalysed: 
GTP = 3',5'-cyclic GMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169668 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanylate cyclase soluble subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 690 amino acids
Theoretical weight: 77.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02108 (Residues: 1-690; Coverage: 100%)
Gene names: GUC1A3, GUCSA3, GUCY1A1, GUCY1A3
Sequence domains:
Guanylate cyclase soluble subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 619 amino acids
Theoretical weight: 70.6 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02153 (Residues: 1-619; Coverage: 100%)
Gene names: GUC1B3, GUCSB3, GUCY1B1, GUCY1B3
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand Z90 1 x Z90
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-30620
Expression system: Spodoptera frugiperda

Quick links

Citations

7 review citations

Carbon monoxide signaling and soluble guanylyl cyclase: Facts, myths, and intriguing possibilities.
Lu et al. (2022)
6 more

1 mention without citation

Vasorelaxant Effects of Syzygium samarangense (Blume) Merr. and L.M.Perry Extract Are Mediated by NO/cGMP Pathway in Isolated Rat Thoracic Aorta.
Hassan et al. (2022)