7d8o

X-ray diffraction
2.1Å resolution

Crystal structure of E. coli ToxIN type III toxin-antitoxin complex

Released:
Source organism: Escherichia coli
Entry authors: Manikandan P, Rothweiler U, Singh M

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Type III toxin-antitoxin system ToxN/AbiQ family toxin Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 187 amino acids
Theoretical weight: 21.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
Antitoxin RNA Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 37 nucleotides
Theoretical weight: 11.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 86.63Å b: 86.643Å c: 123.568Å
α: 90° β: 91.66° γ: 90°
R-values:
R R work R free
0.213 0.213 0.232
Expression system: Escherichia coli BL21(DE3)