7d89

X-ray diffraction
2.89Å resolution

Crystal structure of an inactivated double mutant (E182AE280A) of a novel thermostable GH10 xylanase XynA

Released:
Source organism: Bacillus sp. (in: Bacteria)
Entry authors: Xie W, Yu Q, Wang C

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 49.81 KDa
Source organism: Bacillus sp. (in: Bacteria)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A4P8ESF9 (Residues: 1-408; Coverage: 100%)
Gene name: xyn10A
Sequence domains: Glycosyl hydrolase family 10

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P4122
Unit cell:
a: 110.193Å b: 110.193Å c: 84.983Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.279
Expression system: Escherichia coli BL21(DE3)