› 7d7d

Electron Microscopy
4.5Å resolution

CryoEM structure of gp45-dependent transcription activation complex

Released:
DOI: 10.2210/pdb7d7d/pdb
PDB entry 7d7d coloured by chain, front view.
PDB entry 7d7d coloured by chain, side view.
PDB entry 7d7d coloured by chain, top view.
Source organisms:
Primary publication:
Transcription activation by a sliding clamp.
Shi J, Wen A, Jin S, Gao B, Huang Y, Feng Y
Nat Commun 12 1131 (2021)
PMID: 33602900
Related structures: EMD-30605

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-241161 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli 1-392-07_S4_C3
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
gp55 Chain: F
Molecule details ›
Chain: F
Length: 185 amino acids
Theoretical weight: 21.57 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
Late transcription coactivator Chain: K
Molecule details ›
Chain: K
Length: 132 amino acids
Theoretical weight: 15.01 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P13338 (Residues: 1-112; Coverage: 100%)
Gene name: 33
Sequence domains: Phage late-transcription coactivator
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sliding clamp Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 236 amino acids
Theoretical weight: 25.95 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04525 (Residues: 1-228; Coverage: 100%)
Gene name: 45
Sequence domains:
DNA (template strand) Chain: T
Molecule details ›
Chain: T
Length: 59 nucleotides
Theoretical weight: 18.2 KDa
DNA (nontemplate strand) Chain: N
Molecule details ›
Chain: N
Length: 59 nucleotides
Theoretical weight: 18.16 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-30605
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From Processivity to Genome Maintenance: The Many Roles of Sliding Clamps.
Mulye et al. (2022)
5 other articles