7cyk

X-ray diffraction
2.78Å resolution

Crystal structure of a second cysteine-pair mutant (V110C-I197C) of a bacterial bile acid transporter before disulfide bond formation

Released:
Source organism: Yersinia frederiksenii
Entry authors: Wang X, Lyu Y, Ji Y, Sun Z, Zhou X

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transporter, sodium/bile acid symporter family Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 32.72 KDa
Source organism: Yersinia frederiksenii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A380PV03 (Residues: 1-307; Coverage: 100%)
Gene name: NCTC11470_02445
Sequence domains: Sodium Bile acid symporter family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 190.421Å b: 46.652Å c: 70.781Å
α: 90° β: 101.01° γ: 90°
R-values:
R R work R free
0.228 0.226 0.267
Expression system: Escherichia coli