› 7cvq

X-ray diffraction
3.3Å resolution

crystal structure of Arabidopsis CO CCT domain in complex with NF-YB2/YC3 and FT CORE1 DNA

Released:
DOI: 10.2210/pdb7cvq/pdb
PDB entry 7cvq coloured by chain, front view.
PDB entry 7cvq coloured by chain, side view.
PDB entry 7cvq coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex.
Lv X, Zeng X, Hu H, Chen L, Zhang F, Liu R, Liu Y, Zhou X, Wang C, Wu Z, Kim C, He Y, Du J
Plant Cell 33 1182-1195 (2021)
PMID: 33693873

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121183 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Nuclear transcription factor Y subunit C-3; Zinc finger protein CONSTANS Chains: A, F, K, P
Molecule details ›
Chains: A, F, K, P
Length: 174 amino acids
Theoretical weight: 20.02 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZVL3 (Residues: 55-148; Coverage: 43%)
  • Canonical: Q39057 (Residues: 290-357; Coverage: 18%)
Gene names: At1g54830, At5g15840, CO, F14C21.41, F14F8_220, NFYC3, T22H22.22
Sequence domains:
Nuclear transcription factor Y subunit B-2 Chains: B, G, L, Q
Molecule details ›
Chains: B, G, L, Q
Length: 97 amino acids
Theoretical weight: 11.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9FGJ3 (Residues: 24-120; Coverage: 51%)
Gene names: At5g47640, HAP3B, MNJ7.23, NFYB2
Sequence domains: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
FT CORE1 DNA forward strand Chains: D, I, N, T
Molecule details ›
Chains: D, I, N, T
Length: 25 nucleotides
Theoretical weight: 7.76 KDa
FT CORE1 DNA reverse strand Chains: E, J, O, U
Molecule details ›
Chains: E, J, O, U
Length: 25 nucleotides
Theoretical weight: 7.59 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 103.244Å b: 65.273Å c: 138.284Å
α: 90° β: 91.17° γ: 90°
R-values:
R R work R free
0.242 0.241 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Molecular Genetic Understanding of Photoperiodic Regulation of Flowering Time in Arabidopsis and Soybean.
Luo et al. (2021)
4 more

1 mention without citation

Evolutionary insights and expression dynamics of the CaNFYB transcription factor gene family in pepper (Capsicum annuum) under salinity stress.
Abd El Moneim et al. (2023)