› 7crh

Electron Microscopy
3.3Å resolution

Cryo-EM structure of SKF83959 bound dopamine receptor DRD1-Gs signaling complex

Released:
DOI: 10.2210/pdb7crh/pdb
PDB entry 7crh coloured by chain, front view.
PDB entry 7crh coloured by chain, side view.
PDB entry 7crh coloured by chain, top view.
Source organisms:
Primary publication:
Ligand recognition and allosteric regulation of DRD1-Gs signaling complexes.
Xiao P, Yan W, Gou L, Zhong YN, Kong L, Wu C, Wen X, Yuan Y, Cao S, Qu C, Yang X, Yang CC, Xia A, Hu Z, Zhang Q, He YH, Zhang DL, Zhang C, Hou GH, Liu H, Zhu L, Fu P, Yang S, Rosenbaum DM, Sun JP, Du Y, Zhang L, Yu X, Shao Z
Cell 184 943-956.e18 (2021)
PMID: 33571432
Related structures: EMD-30452

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-210825 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 356 amino acids
Theoretical weight: 39.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 156 amino acids
Theoretical weight: 16.93 KDa
Source organism: Lama glama
Expression system: Escherichia coli
D(1A) dopamine receptor Chain: R
Molecule details ›
Chain: R
Length: 446 amino acids
Theoretical weight: 49.34 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21728 (Residues: 1-446; Coverage: 100%)
Gene name: DRD1
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand GBU 1 x GBU
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-30452
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

7 review citations

Ligands of Adrenergic Receptors: A Structural Point of View.
Wu et al. (2021)
6 more

4 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
3 more