› 7cld

X-ray diffraction
2.61Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-236978 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 450 amino acids
Theoretical weight: 50.04 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-450; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 16.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Tubulin tyrosine ligase Chain: F
Molecule details ›
Chain: F
Length: 384 amino acids
Theoretical weight: 44.38 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli

Ligands and Environments

7 bound ligands:
Ligand GTP 3 x GTP
Ligand MG 5 x MG
Ligand CA 7 x CA
Ligand GDP 1 x GDP
Ligand MES 1 x MES
Ligand G2X 2 x G2X
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 104.422Å b: 160.778Å c: 174.764Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.258
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Microtubule Targeting Agents in Disease: Classic Drugs, Novel Roles.
Wordeman et al. (2021)
1 more

2 mentions without citation

Microtubule-Stabilizing 1,2,4-Triazolo[1,5-a]pyrimidines as Candidate Therapeutics for Neurodegenerative Disease: Matched Molecular Pair Analyses and Computational Studies Reveal New Structure-Activity Insights.
Alle et al. (2023)
1 more