› 7cl8

X-ray diffraction
1.42Å resolution

Testosterone-bound structure of CYP154C2 from Streptomyces avermitilis in an closed conformation

Released:
DOI: 10.2210/pdb7cl8/pdb
PDB entry 7cl8 coloured by chain, front view.
PDB entry 7cl8 coloured by chain, side view.
PDB entry 7cl8 coloured by chain, top view.
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Primary publication:
Improved 2α-Hydroxylation Efficiency of Steroids by CYP154C2 Using Structure-Guided Rational Design.
Gao Q, Ma B, Wang Q, Zhang H, Fushinobu S, Yang J, Lin S, Sun K, Han BN, Xu LH
Appl Environ Microbiol 89 e0218622 (2023)
PMID: 36847541

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 45.27 KDa
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Expression system: Escherichia coli
UniProt:
  • Canonical: Q82GL5 (Residues: 1-400; Coverage: 100%)
Gene names: SAVERM_3882, cyp19
Sequence domains: Cytochrome P450

Ligands and Environments

4 bound ligands:
Ligand HEM 1 x HEM
Ligand PGE 1 x PGE
Ligand PEG 2 x PEG
Ligand TES 1 x TES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Unit cell:
a: 53.74Å b: 61.82Å c: 133.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.161
Expression system: Escherichia coli

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Citations

1 mention without citation

Improved 2α-Hydroxylation Efficiency of Steroids by CYP154C2 Using Structure-Guided Rational Design.
Gao et al. (2023)