› 7ckv

X-ray diffraction
1.63Å resolution

Crystal structure of Cyanobacteriochrome GAF domain in Pr state

Released:
DOI: 10.2210/pdb7ckv/pdb
PDB entry 7ckv coloured by chain, front view.
PDB entry 7ckv coloured by chain, side view.
PDB entry 7ckv coloured by chain, top view.
Source organism: Microchaete diplosiphon
Primary publication:
Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE.
Nagae T, Unno M, Koizumi T, Miyanoiri Y, Fujisawa T, Masui K, Kamo T, Wada K, Eki T, Ito Y, Hirose Y, Mishima M
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33972439

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.39 KDa
Source organism: Microchaete diplosiphon
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q47897 (Residues: 115-273; Coverage: 24%)
Gene name: rcaE
Sequence domains: GAF domain

Ligands and Environments

2 bound ligands:
Ligand CYC 2 x CYC
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: P21
Unit cell:
a: 35.73Å b: 101.334Å c: 41.81Å
α: 90° β: 91.788° γ: 90°
R-values:
R R work R free
0.16 0.158 0.199
Expression system: Escherichia coli BL21

Quick links

Citations

4 citation in other articles

Light- and pH-dependent structural changes in cyanobacteriochrome AnPixJg2.
Altmayer et al. (2022)
3 more

1 mention without citation

Spectroscopic approach for exploring structure and function of photoreceptor proteins.
Unno et al. (2021)