Structure analysis

Cryo-EM structure of the flagellar motor-hook complex from Salmonella

Electron Microscopy
3.9Å resolution
Assembly composition:
hetero 219-mer (preferred)
Entry contents: 16 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 219-mer
PDBe Complex ID: PDB-CPX-140993

Macromolecules

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Chains: 5, 6, 7, 8, 9
Length: 21 amino acids
Theoretical weight: 2 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15928 (Residues: 311-331; Coverage: 4%)
Gene names: STM1969, fla AII.1, fla BI, fliF

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Chains: k, l, m, n, o
Length: 138 amino acids
Theoretical weight: 15.15 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16437 (Residues: 1-138; Coverage: 100%)
Gene names: STM1174, fla FII, flbA, flgB
Pfam: Flagella basal body rod protein
InterPro:

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Chains: q, r, s, t, u, v
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P26462 (Residues: 1-104; Coverage: 100%)
Gene names: STM1968, fla AI, flaN, fliE
Pfam: Flagellar hook-basal body complex protein FliE
InterPro: Flagellar hook-basal body complex protein FliE

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Chain: CE
Length: 264 amino acids
Theoretical weight: 28.94 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P54702 (Residues: 1-264; Coverage: 100%)
Gene names: STM1981, flaP, fliR
Pfam: Bacterial export proteins, family 1
InterPro:

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Chains: CA, CB, CC, CD
Length: 89 amino acids
Theoretical weight: 9.61 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1L5 (Residues: 1-89; Coverage: 100%)
Gene names: STM1980, flaQ, fliQ
Pfam: Bacterial export proteins, family 3
InterPro:

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Chains: CF, w, x, y, z
Length: 245 amino acids
Theoretical weight: 26.8 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P54700 (Residues: 1-245; Coverage: 100%)
Gene names: STM1979, flaR, fliP
Pfam: FliP family
InterPro:

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Chains: Ca, Cb, Cc, Cd, Ce, Cf, Cg, Ch, Ci, Cj, Ck, Cl, Cm, Cn, Co, Cp, Cq, Cr, Cs, Ct, Cu, Cv, Cw, Cx, Cy, Cz, Da, Db, Dc, Dd, De, Df, Dg, Dh, Di, Dj, Dk, Dl, Dm, Dn, Do, Dp, Dq, Dr, Ds, Dt, Du, Dv, Dw, Ea, Eb, Ec, Ed, Ee, Ef, Eg, Eh
Length: 560 amino acids
Theoretical weight: 61.3 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15928 (Residues: 1-560; Coverage: 100%)
Gene names: STM1969, fla AII.1, fla BI, fliF
Pfam:
InterPro:

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Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ
Length: 232 amino acids
Theoretical weight: 24.97 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1N8 (Residues: 1-232; Coverage: 100%)
Gene names: STM1180, fla FVIII, flaY, flgH
Pfam: Flagellar L-ring protein
InterPro: Flagellar L-ring protein

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Chains: BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ
Length: 365 amino acids
Theoretical weight: 38.19 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15930 (Residues: 1-365; Coverage: 100%)
Gene names: STM1181, fla FIX, flaM, flgI
Pfam: Flagellar P-ring protein
InterPro: Flagellar P-ring protein

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