7cgo

Electron Microscopy
3.9Å resolution

Cryo-EM structure of the flagellar motor-hook complex from Salmonella

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 219-mer (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Flagellar basal-body rod protein FlgG Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 260 amino acids
Theoretical weight: 27.78 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1J3 (Residues: 1-260; Coverage: 100%)
Gene names: STM1179, fla FVII, flaL, flgG
Sequence domains: Flagellar basal body rod FlgEFG protein C-terminal
Flagellar basal-body rod protein FlgF Chains: a, b, c, d, e
Molecule details ›
Chains: a, b, c, d, e
Length: 251 amino acids
Theoretical weight: 26.12 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16323 (Residues: 1-251; Coverage: 100%)
Gene names: STM1178, fla FVI, flaX, flgF
Sequence domains: Flagellar basal body rod FlgEFG protein C-terminal
Flagellar MS ring L2 Chains: 0, 1, 2, 3, 4
Molecule details ›
Chains: 0, 1, 2, 3, 4
Length: 15 amino acids
Theoretical weight: 1.29 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Flagellar M-ring protein Chains: 5, 6, 7, 8, 9
Molecule details ›
Chains: 5, 6, 7, 8, 9
Length: 21 amino acids
Theoretical weight: 2 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15928 (Residues: 311-331; Coverage: 4%)
Gene names: STM1969, fla AII.1, fla BI, fliF
Flagellar basal-body rod protein FlgC Chains: f, g, h, i, j, p
Molecule details ›
Chains: f, g, h, i, j, p
Length: 134 amino acids
Theoretical weight: 13.99 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1I7 (Residues: 1-134; Coverage: 100%)
Gene names: STM1175, fla FIII, flaW, flgC
Sequence domains: Flagellar basal body rod FlgEFG protein C-terminal
Flagellar basal body rod protein FlgB Chains: k, l, m, n, o
Molecule details ›
Chains: k, l, m, n, o
Length: 138 amino acids
Theoretical weight: 15.15 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P16437 (Residues: 1-138; Coverage: 100%)
Gene names: STM1174, fla FII, flbA, flgB
Flagellar hook-basal body complex protein FliE Chains: q, r, s, t, u, v
Molecule details ›
Chains: q, r, s, t, u, v
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P26462 (Residues: 1-104; Coverage: 100%)
Gene names: STM1968, fla AI, flaN, fliE
Sequence domains: Flagellar hook-basal body complex protein FliE
Flagellar hook protein FlgE Chains: DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK, DL, DM, DN, DO, DP, DQ, DR, DS, DT, DU, DV, DW, DX, DY, DZ, EA, EB, EC, ED, EE, EF, EG
Molecule details ›
Chains: DA, DB, DC, DD, DE, DF, DG, DH, DI, DJ, DK, DL, DM, DN, DO, DP, DQ, DR, DS, DT, DU, DV, DW, DX, DY, DZ, EA, EB, EC, ED, EE, EF, EG
Length: 403 amino acids
Theoretical weight: 42.23 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1J1 (Residues: 1-403; Coverage: 100%)
Gene names: STM1177, fla FV, flaK, flgE
Sequence domains:
Flagellar biosynthetic protein FliR Chain: CE
Molecule details ›
Chain: CE
Length: 264 amino acids
Theoretical weight: 28.94 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P54702 (Residues: 1-264; Coverage: 100%)
Gene names: STM1981, flaP, fliR
Sequence domains: Bacterial export proteins, family 1
Flagellar biosynthetic protein FliQ Chains: CA, CB, CC, CD
Molecule details ›
Chains: CA, CB, CC, CD
Length: 89 amino acids
Theoretical weight: 9.61 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1L5 (Residues: 1-89; Coverage: 100%)
Gene names: STM1980, flaQ, fliQ
Sequence domains: Bacterial export proteins, family 3
Flagellar biosynthetic protein FliP Chains: CF, w, x, y, z
Molecule details ›
Chains: CF, w, x, y, z
Length: 245 amino acids
Theoretical weight: 26.8 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P54700 (Residues: 1-245; Coverage: 100%)
Gene names: STM1979, flaR, fliP
Sequence domains: FliP family
Flagellar M-ring protein Chains: Ca, Cb, Cc, Cd, Ce, Cf, Cg, Ch, Ci, Cj, Ck, Cl, Cm, Cn, Co, Cp, Cq, Cr, Cs, Ct, Cu, Cv, Cw, Cx, Cy, Cz, Da, Db, Dc, Dd, De, Df, Dg, Dh, Di, Dj, Dk, Dl, Dm, Dn, Do, Dp, Dq, Dr, Ds, Dt, Du, Dv, Dw, Ea, Eb, Ec, Ed, Ee, Ef, Eg, Eh
Molecule details ›
Chains: Ca, Cb, Cc, Cd, Ce, Cf, Cg, Ch, Ci, Cj, Ck, Cl, Cm, Cn, Co, Cp, Cq, Cr, Cs, Ct, Cu, Cv, Cw, Cx, Cy, Cz, Da, Db, Dc, Dd, De, Df, Dg, Dh, Di, Dj, Dk, Dl, Dm, Dn, Do, Dp, Dq, Dr, Ds, Dt, Du, Dv, Dw, Ea, Eb, Ec, Ed, Ee, Ef, Eg, Eh
Length: 560 amino acids
Theoretical weight: 61.3 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15928 (Residues: 1-560; Coverage: 100%)
Gene names: STM1969, fla AII.1, fla BI, fliF
Sequence domains:
FlgB-Dc loop Chains: GA, GB, GC, GD, GE
Molecule details ›
Chains: GA, GB, GC, GD, GE
Length: 12 amino acids
Theoretical weight: 1.04 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
FliE helix 1 Chains: GF, GG, GH, GI, GJ, GK
Molecule details ›
Chains: GF, GG, GH, GI, GJ, GK
Length: 18 amino acids
Theoretical weight: 1.55 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Flagellar L-ring protein Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ
Molecule details ›
Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ
Length: 232 amino acids
Theoretical weight: 24.97 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P0A1N8 (Residues: 1-232; Coverage: 100%)
Gene names: STM1180, fla FVIII, flaY, flgH
Sequence domains: Flagellar L-ring protein
Flagellar P-ring protein Chains: BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ
Molecule details ›
Chains: BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ
Length: 365 amino acids
Theoretical weight: 38.19 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
UniProt:
  • Canonical: P15930 (Residues: 1-365; Coverage: 100%)
Gene names: STM1181, fla FIX, flaM, flgI
Sequence domains: Flagellar P-ring protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-30359