7cgf

X-ray diffraction
1.7Å resolution

Crystal Structure of PUF-8 in Complex with PBE-RNA

Released:
Source organism: Caenorhabditis elegans
Primary publication:
Structural recognition of the mRNA 3' UTR by PUF-8 restricts the lifespan of C. elegans.
Nucleic Acids Res 49 10082-10097 (2021)
PMID: 34478557

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
PUM-HD domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 41.91 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q09487 (Residues: 172-522; Coverage: 66%)
Gene names: C30G12.7, CELE_C30G12.7, puf-8
PBE-5G (5'-R(P*UP*GP*UP*AP*GP*AP*UP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.55 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P41212
Unit cell:
a: 89.564Å b: 89.564Å c: 117.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.221
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided