7ce1

X-ray diffraction
3.2Å resolution

Complex STRUCTURE OF TRANSCRIPTION FACTOR SghR with its COGNATE DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-104253 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH lacI-type domain-containing protein Chains: A, B, C, D, G, H, K, L, O, P, S, T
Molecule details ›
Chains: A, B, C, D, G, H, K, L, O, P, S, T
Length: 341 amino acids
Theoretical weight: 37.97 KDa
Source organism: Agrobacterium tumefaciens A6
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A2I4PGE9 (Residues: 18-350; Coverage: 95%)
Gene name: sghR
Sequence domains:
promoter DNA Chains: a, b, c, d, g, h, k, l, o, p, s, t
Molecule details ›
Chains: a, b, c, d, g, h, k, l, o, p, s, t
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 107.9Å b: 218.96Å c: 284.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.281
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'