› 7cai

Electron Microscopy
3.49Å resolution

SARS-CoV-2 S trimer with two RBDs in the open state and complexed with two H014 Fab

Released:
DOI: 10.2210/pdb7cai/pdb
PDB entry 7cai coloured by chain, front view.
PDB entry 7cai coloured by chain, side view.
PDB entry 7cai coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for neutralization of SARS-CoV-2 and SARS-CoV by a potent therapeutic antibody.
Lv Z, Deng YQ, Ye Q, Cao L, Sun CY, Fan C, Huang W, Sun S, Sun Y, Zhu L, Chen Q, Wang N, Nie J, Cui Z, Zhu D, Shaw N, Li XF, Li Q, Xie L, Wang Y, Rao Z, Qin CF, Wang X
Science 369 1505-1509 (2020)
PMID: 32703908
Related structures: EMD-30332

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Spike glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1208 amino acids
Theoretical weight: 133.87 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1208; Coverage: 95%)
Gene names: 2, S
Sequence domains:
Light chain of H014 Fab Chains: D, F
Molecule details ›
Chains: D, F
Length: 210 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Heavy chain of H014 Fab Chains: E, G
Molecule details ›
Chains: E, G
Length: 223 amino acids
Theoretical weight: 23.82 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 30 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.49Å
Relevant EMDB volumes: EMD-30332
Expression system: Homo sapiens

Quick links

Citations

82 review citations

The SARS-CoV-2 Spike Glycoprotein Biosynthesis, Structure, Function, and Antigenicity: Implications for the Design of Spike-Based Vaccine Immunogens.
Duan et al. (2020)
81 more

18 mentions without citation

Protective neutralizing epitopes in SARS-CoV-2.
Liu et al. (2022)
17 more