› 7c85

X-ray diffraction
1.75Å resolution

Esterase AlinE4 mutant-S13A

Released:
DOI: 10.2210/pdb7c85/pdb
PDB entry 7c85 coloured by chain, front view.
PDB entry 7c85 coloured by chain, side view.
PDB entry 7c85 coloured by chain, top view.
Source organism: Altericroceibacterium indicum
Primary publication:
Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases.
Li Z, Li L, Huo Y, Chen Z, Zhao Y, Huang J, Jian S, Rong Z, Wu D, Gan J, Hu X, Li J, Xu XW
Biotechnol Biofuels 13 107 (2020)
PMID: 32549911

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SGNH hydrolase-type esterase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 20.69 KDa
Source organism: Altericroceibacterium indicum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A4P1LYH7 (Residues: 1-191; Coverage: 100%)
Sequence domains: GDSL-like Lipase/Acylhydrolase family

Ligands and Environments

2 bound ligands:
Ligand CD 6 x CD
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P42212
Unit cell:
a: 79.81Å b: 79.81Å c: 60.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.121 0.177
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Thioesterase enzyme families: Functions, structures, and mechanisms.
Caswell et al. (2022)
2 more

1 mention without citation

Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases.
Li et al. (2020)