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› 7c6d

X-ray diffraction
1.45Å resolution

Crystal structure of E19A mutant chitosanase from Bacillus subtilis MY002 complexed with 6 GlcN.

Released:
DOI: 10.2210/pdb7c6d/pdb
PDB entry 7c6d coloured by chain, front view.
PDB entry 7c6d coloured by chain, side view.
PDB entry 7c6d coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structure-based rational design of chitosanase CsnMY002 for high yields of chitobiose.
Li Y, Gou Y, Liu Z, Xie T, Wang G
Colloids Surf B Biointerfaces 202 111692 (2021)
PMID: 33744813

Function and Biology Details

Reaction catalysed: 
[Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues ina partly acetylated chitosan.]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitosanase Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.41 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O07921 (Residues: 36-277; Coverage: 100%)
Gene names: BSU26890, csn
Sequence domains: Glycosyl hydrolase family 46

Ligands and Environments

Carbohydrate polymer : NEW Components: GCS
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 46.961Å b: 77.921Å c: 79.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.136 0.16
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biodegradation and Prospect of Polysaccharide from Crustaceans.
Qiu et al. (2022)
2 more

1 mention without citation

Heterologous Expression and Characterization of a High-Efficiency Chitosanase From Bacillus mojavensis SY1 Suitable for Production of Chitosan Oligosaccharides.
Wang et al. (2021)