› 7c61

X-ray diffraction
3Å resolution

Crystal structure of 5-HT1B-BRIL and SRP2070_Fab complex

Released:
DOI: 10.2210/pdb7c61/pdb
PDB entry 7c61 coloured by chain, front view.
PDB entry 7c61 coloured by chain, side view.
PDB entry 7c61 coloured by chain, top view.
Source organisms:
Primary publication:
The discovery of a new antibody for BRIL-fused GPCR structure determination.
Miyagi H, Asada H, Suzuki M, Takahashi Y, Yasunaga M, Suno C, Iwata S, Saito JI
Sci Rep 10 11669 (2020)
PMID: 32669569

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209525 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
IGG LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.68 KDa
Source organism: Mus musculus
Expression system: Mus musculus
IGG HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 323 amino acids
Theoretical weight: 35.24 KDa
Source organism: Mus musculus
Expression system: Mus musculus
5-hydroxytryptamine receptor 1B; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 45.11 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28222 (Residues: 33-239, 306-390; Coverage: 75%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: HTR1B, HTR1DB, cybC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ERM 1 x ERM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 248.8Å b: 65.7Å c: 79.73Å
α: 90° β: 98.79° γ: 90°
R-values:
R R work R free
0.254 0.252 0.29
Expression systems:
  • Mus musculus
  • Spodoptera frugiperda

Quick links

Citations

4 review citations

Structural insights into ligand recognition and activation of angiotensin receptors.
Zhang et al. (2021)
3 more

6 mentions without citation

Structure, dynamics and lipid interactions of serotonin receptors: excitements and challenges.
Sarkar et al. (2020)
5 more