› 7c1u

X-ray diffraction
1.4Å resolution

Crystal structure of the starter condensation domain of rhizomide synthetase RzmA mutant H140V/R148A in a "product-released" conformation

Released:
DOI: 10.2210/pdb7c1u/pdb
PDB entry 7c1u coloured by chain, front view.
PDB entry 7c1u coloured by chain, side view.
PDB entry 7c1u coloured by chain, top view.
Source organism: Mycetohabitans rhizoxinica HKI 454
Primary publication:
Engineering and elucidation of the lipoinitiation process in nonribosomal peptide biosynthesis.
Zhong L, Diao X, Zhang N, Li F, Zhou H, Chen H, Bai X, Ren X, Zhang Y, Wu D, Bian X
Nat Commun 12 296 (2021)
PMID: 33436600

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carrier domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 48.42 KDa
Source organism: Mycetohabitans rhizoxinica HKI 454
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E5ATN9 (Residues: 1-431; Coverage: 6%)
Gene name: RBRH_01504
Sequence domains: Condensation domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 52.564Å b: 58.784Å c: 134.058Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains.
Corpuz et al. (2022)
3 more

1 mention without citation

Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases.
Patel et al. (2023)