› 7bwx

X-ray diffraction
1.9Å resolution

Crystal structure of ice-binding protein from an Antarctic ascomycete, Antarctomyces psychrotrophicus.

Released:
DOI: 10.2210/pdb7bwx/pdb
PDB entry 7bwx coloured by chain, front view.
PDB entry 7bwx coloured by chain, side view.
PDB entry 7bwx coloured by chain, top view.
Source organism: Antarctomyces psychrotrophicus
Primary publication:
An Ice-Binding Protein from an Antarctic Ascomycete Is Fine-Tuned to Bind to Specific Water Molecules Located in the Ice Prism Planes.
Yamauchi A, Arai T, Kondo H, Sasaki YC, Tsuda S
Biomolecules 10 (2020)
PMID: 32414092

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ice-binding protein isoform1a Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 222 amino acids
Theoretical weight: 22.28 KDa
Source organism: Antarctomyces psychrotrophicus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A2Z6DSM4 (Residues: 21-235; Coverage: 100%)
Gene name: ibp
Sequence domains: Ice-binding-like

Ligands and Environments

2 bound ligands:
Ligand SO4 12 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21212
Unit cell:
a: 86.427Å b: 207.281Å c: 100.784Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.135 0.164
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Antifreeze Proteins: A Tale of Evolution From Origin to Energy Applications.
Gharib et al. (2021)
3 other articles

1 mention without citation

An Ice-Binding Protein from an Antarctic Ascomycete Is Fine-Tuned to Bind to Specific Water Molecules Located in the Ice Prism Planes.
Yamauchi et al. (2020)