› 7bus

X-ray diffraction
2.52Å resolution

Chalcone synthase from Glycine max (L.) Merr (soybean)

Released:
DOI: 10.2210/pdb7bus/pdb
PDB entry 7bus coloured by chain, front view.
PDB entry 7bus coloured by chain, side view.
PDB entry 7bus coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Crystal structure of chalcone synthase, a key enzyme for isoflavonoid biosynthesis in soybean.
Imaizumi R, Mameda R, Takeshita K, Kubo H, Sakai N, Nakata S, Takahashi S, Kataoka K, Yamamoto M, Nakayama T, Yamashita S, Waki T
Proteins (2020)
PMID: 32725893

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 44.65 KDa
Source organism: Glycine max
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P24826 (Residues: 1-388; Coverage: 100%)
Gene name: CHS1
Sequence domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand SME 2 x SME
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P212121
Unit cell:
a: 54.76Å b: 98.94Å c: 159.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.189 0.254
Expression system: Escherichia coli BL21

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Citations

1 review citation

Comparative Molecular Mechanisms of Biosynthesis of Naringenin and Related Chalcones in Actinobacteria and Plants: Relevance for the Obtention of Potent Bioactive Metabolites.
Martín et al. (2022)
3 other articles