› 7bts

X-ray diffraction
3.13Å resolution

Structure of human beta1 adrenergic receptor bound to epinephrine and nanobody 6B9

Released:
DOI: 10.2210/pdb7bts/pdb
PDB entry 7bts coloured by chain, front view.
PDB entry 7bts coloured by chain, side view.
PDB entry 7bts coloured by chain, top view.
Source organisms:
Primary publication:
Binding pathway determines norepinephrine selectivity for the human β1AR over β2AR.
Xu X, Kaindl J, Clark MJ, Hübner H, Hirata K, Sunahara RK, Gmeiner P, Kobilka BK, Liu X
Cell Res 31 569-579 (2021)
PMID: 33093660

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207763 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-1 adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 52.24 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: D9IEF7 (Residues: 2-161; Coverage: 98%)
  • Canonical: P08588 (Residues: 54-399; Coverage: 61%)
Gene names: ADRB1, ADRB1R, B1AR, T4Tp126, e
Sequence domains:
Camelid Antibody Fragment Chain: B
Molecule details ›
Chain: B
Length: 120 amino acids
Theoretical weight: 12.95 KDa
Source organism: Vicugna pacos
Expression system: Spodoptera frugiperda

Ligands and Environments

6 bound ligands:
Ligand ALE 1 x ALE
Ligand SO4 6 x SO4
Ligand NA 1 x NA
Ligand EPE 1 x EPE
Ligand CLR 1 x CLR
Ligand 1WV 1 x 1WV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 377.98Å b: 66.22Å c: 47.86Å
α: 90° β: 93.883° γ: 90°
R-values:
R R work R free
0.237 0.236 0.258
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

Short-Chain Fatty Acid Receptors and Cardiovascular Function.
Lymperopoulos et al. (2022)
10 more

5 mentions without citation

Phenylalanine 193 in Extracellular Loop 2 of the β 2-Adrenergic Receptor Coordinates β-Arrestin Interaction.
Ippolito et al. (2022)
4 more