› 7btc

X-ray diffraction
2.1Å resolution

Crystal structure of Rheb Y35N mutant bound to GDP

Released:
DOI: 10.2210/pdb7btc/pdb
PDB entry 7btc coloured by chain, front view.
PDB entry 7btc coloured by chain, side view.
PDB entry 7btc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the functions of dominantly active Y35N and inactive D60K Rheb mutants in mTORC1 signaling.
Zhang C, Liu Y, Zhang Y, Wang X, Zhang T, Ding J
J Mol Cell Biol 12 741-744 (2020)
PMID: 32470140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein Rheb Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 177 amino acids
Theoretical weight: 20.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15382 (Residues: 1-169; Coverage: 92%)
Gene names: RHEB, RHEB2
Sequence domains: Ras family

Ligands and Environments

1 bound ligand:
Ligand GDP 4 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 75.426Å b: 78.656Å c: 117.398Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Molecular basis for the functions of dominantly active Y35N and inactive D60K Rheb mutants in mTORC1 signaling.
Zhang et al. (2020)