› 7brt

X-ray diffraction
2Å resolution

Crystal structure of Sec62 LIR fused to GABARAP

Released:
DOI: 10.2210/pdb7brt/pdb
PDB entry 7brt coloured by chain, front view.
PDB entry 7brt coloured by chain, side view.
PDB entry 7brt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes.
Mochida K, Yamasaki A, Matoba K, Kirisako H, Noda NN, Nakatogawa H
Nat Commun 11 3306 (2020)
PMID: 32620754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-aminobutyric acid receptor-associated protein; Translocation protein SEC62 Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99442 (Residues: 361-376; Coverage: 4%)
  • Canonical: O95166 (Residues: 1-116; Coverage: 99%)
Gene names: FLC3B, GABARAP, HT004, SEC62, TLOC1
Sequence domains: Autophagy protein Atg8 ubiquitin like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 42.887Å b: 42.889Å c: 144.012Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

ER-Phagy, ER Homeostasis, and ER Quality Control: Implications for Disease.
Ferro-Novick et al. (2021)
9 more