› 7bpc

X-ray diffraction
2.45Å resolution

Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum in complex with 2,5-DHBA

Released:
DOI: 10.2210/pdb7bpc/pdb
PDB 7bpc coloured by chain and viewed from the front.
PDB 7bpc coloured by chain and viewed from the side.
PDB 7bpc coloured by chain and viewed from the top.
Source organism: Fusarium oxysporum
Primary publication:
2,3-Dihydroxybenzoic Acid Decarboxylase from Fusarium oxysporum: Crystal Structures and Substrate Recognition Mechanism.
Song M, Zhang X, Liu W, Feng J, Cui Y, Yao P, Wang M, Guo RT, Wu Q, Zhu D
Chembiochem 21 2950-2956 (2020)
PMID: 32421914

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydro-rel domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 343 amino acids
Theoretical weight: 39.29 KDa
Source organism: Fusarium oxysporum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: N1S495 (Residues: 1-336; Coverage: 100%)
Gene name: FOC4_g10002133
Sequence domains: Amidohydrolase

Ligands and Environments

2 bound ligands:
Ligand GTQ 3 x GTQ
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P212121
Unit cell:
a: 76.769Å b: 129.739Å c: 140.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.247
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Bright Side of Fusarium oxysporum: Secondary Metabolites Bioactivities and Industrial Relevance in Biotechnology and Nanotechnology.
Ibrahim et al. (2021)
1 more