› 7bl7

X-ray diffraction
3.33Å resolution

Crystal structure of UMPK from M. tuberculosis in complex with UDP and UTP (P21212 form)

Released:
DOI: 10.2210/pdb7bl7/pdb
PDB entry 7bl7 coloured by chain, front view.
PDB entry 7bl7 coloured by chain, side view.
PDB entry 7bl7 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural basis for the allosteric inhibition of UMP kinase from Gram-positive bacteria, a promising antibacterial target.
Walter P, Mechaly A, Bous J, Haouz A, England P, Lai-Kee-Him J, Ancelin A, Hoos S, Baron B, Trapani S, Bron P, Labesse G, Munier-Lehmann H
FEBS J (2022)
PMID: 35152545

Function and Biology Details

Reaction catalysed: 
ATP + UMP = ADP + UDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 281 amino acids
Theoretical weight: 29.63 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHK5 (Residues: 1-261; Coverage: 100%)
Gene names: MTCY274.14c, Rv2883c, pyrH
Sequence domains: Amino acid kinase family

Ligands and Environments

2 bound ligands:
Ligand UDP 8 x UDP
Ligand UTP 8 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 151.23Å b: 165.75Å c: 125.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.274
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Genetic diversity revealed by single nucleotide polymorphism markers in a worldwide germplasm collection of durum wheat.
Ren et al. (2013)