7bl7

X-ray diffraction
3.33Å resolution

Crystal structure of UMPK from M. tuberculosis in complex with UDP and UTP (P21212 form)

Released:
Entry authors: Walter P, Labesse G, Haouz A, Mechaly AE, Munier-Lehmann H

Function and Biology Details

Reaction catalysed:
ATP + UMP = ADP + UDP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 281 amino acids
Theoretical weight: 29.63 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHK5 (Residues: 1-261; Coverage: 100%)
Gene names: MTCY274.14c, Rv2883c, pyrH
Sequence domains: Amino acid kinase family

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 151.23Å b: 165.75Å c: 125.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.274
Expression system: Escherichia coli