› 7biz

X-ray diffraction
1.53Å resolution

Structure of a B12 binding lipoprotein from Bacteroides thetaiotaomicron

Released:
DOI: 10.2210/pdb7biz/pdb
PDB 7biz coloured by chain and viewed from the front.
PDB 7biz coloured by chain and viewed from the side.
PDB 7biz coloured by chain and viewed from the top.
Source organism: Bacteroides thetaiotaomicron
Primary publication:
Gut Commensal Bacteroidetes Encode a Novel Class of Vitamin B12-Binding Proteins.
Putnam EE, Abellon-Ruiz J, Killinger BJ, Rosnow JJ, Wexler AG, Folta-Stogniew E, Wright AT, van den Berg B, Goodman AL
mBio e0284521 (2022)
PMID: 35227073

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell surface protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 501 amino acids
Theoretical weight: 56.15 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0N7IAT3 (Residues: 103-593; Coverage: 83%)
Gene names: BSIG_2898, Btheta7330_04243, DW011_18555, EH213_03063, GAN75_24345, GAN91_25070, GAO00_20505, GAO51_14515
Sequence domains: PKD-like domain

Ligands and Environments

5 bound ligands:
Ligand CNC 2 x CNC
Ligand CA 4 x CA
Ligand 15P 3 x 15P
Ligand NA 2 x NA
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3121
Unit cell:
a: 128.052Å b: 128.052Å c: 135.051Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.192
Expression system: Escherichia coli

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