7bgy

Electron Microscopy
2.9Å resolution

Cryo-EM Structure of KdpFABC in E2Pi state with MgF4

Released:
Source organism: Escherichia coli K-12
Entry authors: Sweet ME, Larsen C, Pedersen BP, Stokes DL
Related structures: EMD-12184

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + K(+)(Side 1) = ADP + phosphate + K(+)(Side 2)

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Potassium-transporting ATPase potassium-binding subunit Chain: A
Molecule details ›
Chain: A
Length: 557 amino acids
Theoretical weight: 59.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03959 (Residues: 1-557; Coverage: 100%)
Gene names: JW0686, b0698, kdpA
Sequence domains: Potassium-transporting ATPase A subunit
Potassium-transporting ATPase ATP-binding subunit Chain: B
Molecule details ›
Chain: B
Length: 682 amino acids
Theoretical weight: 72.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03960 (Residues: 1-682; Coverage: 100%)
Gene names: JW0685, b0697, kdpB
Sequence domains:
Potassium-transporting ATPase KdpC subunit Chain: C
Molecule details ›
Chain: C
Length: 208 amino acids
Theoretical weight: 22.3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03961 (Residues: 1-190; Coverage: 100%)
Gene names: JW0684, b0696, kdpC
Sequence domains: K+-transporting ATPase, c chain
Potassium-transporting ATPase KdpF subunit Chain: D
Molecule details ›
Chain: D
Length: 29 amino acids
Theoretical weight: 3.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P36937 (Residues: 1-29; Coverage: 100%)
Gene names: JW0687, b4513, kdpF
Sequence domains: F subunit of K+-transporting ATPase (Potass_KdpF)

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-12184
Expression system: Escherichia coli