› 7bd0

X-ray diffraction
1.06Å resolution

The adduct of NAMI-A with Hen Egg White Lysozyme at 26 hours.

Released:
DOI: 10.2210/pdb7bd0/pdb
PDB entry 7bd0 coloured by chain, front view.
PDB entry 7bd0 coloured by chain, side view.
PDB entry 7bd0 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Insights into the Protein Ruthenation Mechanism by Antimetastatic Metallodrugs: High-Resolution X-ray Structures of the Adduct Formed between Hen Egg-White Lysozyme and
Chiniadis L, Giastas P, Bratsos I, Papakyriakou A
Inorg Chem 60 10729-10737 (2021)
PMID: 34197115

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

5 bound ligands:
Ligand EDO 2 x EDO
Ligand NA 3 x NA
Ligand IMD 3 x IMD
Ligand RU 3 x RU
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P43212
Unit cell:
a: 78.298Å b: 78.298Å c: 37.571Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.185
Expression system: Gallus gallus

Quick links

Citations

1 review citation

Advantageous Reactivity of Unstable Metal Complexes: Potential Applications of Metal-Based Anticancer Drugs for Intratumoral Injections.
Levina et al. (2022)