7bca

X-ray diffraction
2.8Å resolution

Crystal structure of the HTH DNA binding protein ArdK from R388 plasmid bound to a direct-repeat DNA element

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TrfB transcriptional repressor protein domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.21 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6I6B7 (Residues: 11-107; Coverage: 91%)
Sequence domains: TrfB plasmid transcriptional repressor
DNA (5'-D(*TP*GP*TP*CP*AP*AP*TP*AP*GP*GP*TP*GP*TP*CP*AP*AP*TP*AP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
DNA (5'-D(*GP*TP*AP*TP*TP*GP*AP*CP*AP*CP*CP*TP*AP*TP*TP*GP*AP*CP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 88.12Å b: 39.06Å c: 120.04Å
α: 90° β: 101.65° γ: 90°
R-values:
R R work R free
0.234 0.232 0.293
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided