› 7b7c

X-ray diffraction
1.55Å resolution

Room temperature X-ray structure of perdeuterated PLL lectin in complex with L-fucose

Released:
DOI: 10.2210/pdb7b7c/pdb
PDB 7b7c coloured by chain and viewed from the front.
PDB 7b7c coloured by chain and viewed from the side.
PDB 7b7c coloured by chain and viewed from the top.
Source organism: Photorhabdus laumondii
Primary publication:
Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions.
Gajdos L, Blakeley MP, Kumar A, Wimmerová M, Haertlein M, Forsyth VT, Imberty A, Devos JM
Structure 29 1003-1013.e4 (2021)
PMID: 33765407

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PLL lectin Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 41.94 KDa
Source organism: Photorhabdus laumondii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7N8J0 (Residues: 8-375; Coverage: 98%)
Gene name: plu0732

Ligands and Environments

2 bound ligands:
Ligand FUL 4 x FUL
Ligand FUC 2 x FUC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I222
Unit cell:
a: 72.62Å b: 89.07Å c: 159.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.162
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Neutron crystallography reveals mechanisms used by Pseudomonas aeruginosa for host-cell binding.
Gajdos et al. (2022)
1 more