› 7b6l

X-ray diffraction
2.08Å resolution

Crystal structure of MurE from E.coli in complex with Z57299368

Released:
DOI: 10.2210/pdb7b6l/pdb
PDB entry 7b6l coloured by chain, front view.
PDB entry 7b6l coloured by chain, side view.
PDB entry 7b6l coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Koekemoer L, Steindel M, Fairhead M, Arrowsmith CH, Edwards AM, Bountra C, von Delft F, Krojer T, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 496 amino acids
Theoretical weight: 53.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22188 (Residues: 1-495; Coverage: 100%)
Gene names: JW0083, b0085, murE
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand IPA 3 x IPA
Ligand SZK 2 x SZK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 58.268Å b: 58.147Å c: 74.463Å
α: 96.83° β: 91.42° γ: 104.56°
R-values:
R R work R free
0.209 0.207 0.244
Expression system: Escherichia coli BL21(DE3)

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