› 7b4v

X-ray diffraction
1.4Å resolution

Broadly neutralizing DARPin bnD.2 in complex with the HIV-1 envelope variable loop 3 crown mimetic peptide V3-IF (BG505)

Released:
DOI: 10.2210/pdb7b4v/pdb
PDB 7b4v coloured by chain and viewed from the front.
PDB 7b4v coloured by chain and viewed from the side.
PDB 7b4v coloured by chain and viewed from the top.
Source organism: synthetic construct
Primary publication:
Distinct conformations of the HIV-1 V3 loop crown are targetable for broad neutralization.
Friedrich N, Stiegeler E, Glögl M, Lemmin T, Hansen S, Kadelka C, Wu Y, Ernst P, Maliqi L, Foulkes C, Morin M, Eroglu M, Liechti T, Ivan B, Reinberg T, Schaefer JV, Karakus U, Ursprung S, Mann A, Rusert P, Kouyos RD, Robinson JA, Günthard HF, Plückthun A, Trkola A
Nat Commun 12 6705 (2021)
PMID: 34795280

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Broadly neutralizing DARPin bnD.2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 132 amino acids
Theoretical weight: 13.74 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
HIV-1 envelope variable loop 3 crown mimetic peptide V3-IF (BG505) Chains: B, D
Molecule details ›
Chains: B, D
Length: 15 amino acids
Theoretical weight: 1.6 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CL 5 x CL
Ligand MG 4 x MG
1 modified residue:
Ligand DPR 2 x DPR

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 50.63Å b: 69.34Å c: 69.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.196
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Neutralization Sensitivity of HIV-1 CRF07_BC From an Untreated Patient With a Focus on Evolution Over Time.
Wang et al. (2022)